Linking periodontal pathogens with endoperio lesions

Endodontic-periodontal diseases pose difficulties for the practitioner in diagnosing and predicting the success of the affected teeth. Therefore, it is of interest to correlate between periodontal infections and endodontic periodontal disorders. 50 patients of both sexes were included in this study. 28 of the 50 patients were men and 22 were women. Participants with a history of endodontic and periodontal lesions on the same tooth were chosen. A polymerase chain reaction experiment was carried out and relationships were formed. Data shows that isolates of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans and Tannerella forsythia were identified in 91% of the periodontium, 12% of the endodontium, and 51% of the endodontium, respectively. Targeted bacterial species were associated with periodontal and endodontic disorders that occurred concurrently. Therefore, it is plausible to speculate that dentinal tubules serve as a channel for the dissemination of microorganisms.


Background:
It is difficult for the practitioners to diagnose and predict the success of the affected teeth with endodontic-periodontal diseases.Periodontal tissues and dental pulp have has close association.The development and evolution of such lesions are significantly influenced by a number of contributing variables, including trauma, perforations, root resorptions, and dental abnormalities, as well as etiologic factors including fungi, bacteria, and viruses [1,2].In regions without cementum, exposed dentinal tubules may function as a connecting route between the periodontal ligament and the pulp [1].There may also be a direct line of contact between the pulp and the periodontium through the accessory canals in the furcation area of molar.The main channel of contact between the pulp and the periodontium is the apical foramen [1, 3].It has been found that bacteria can enter through dentinal tubules, and the endodonticperiodontal pathway niche is susceptible to endodontic infection Therefore, it is of interest to explore the correlation between periodontal infections and endodontic periodontal disorders.

Materials and Method:
The Department of Periodontology and Endodontics carried out this investigation.The institutional ethical committee granted ethical approval.Written informed consent was obtained from each patient.Fifty patients, aged 25-55, with a single rooted tooth exhibiting endodontic or periodontal disease, and patients with periodontal pockets less than 6 mm, were included in this research.Participants who had a history of antibiotic usage within the last three months, had an aggressive case of periodontitis, had radiographic evidence of endodonticperiodontal contact, or had a fistula were excluded.Patient information was entered into case history performa, including name, age, gender, and other details.To diagnose endodonticperiodontal lesions, intraoral periapical radiographs were performed on each patient.Two to three paper points were placed into periodontal pockets and left there for one minute each in order to obtain periodontal samples.Two to three sterile paper points were placed into the root canal and left there for one minute in order to collect samples from a tooth that had a single root canal.After that, the paper points were put into cryotubes with one millilitre of TE buffer inside of them.Samples were frozen right away and stored at -80°C.

Extraction of DNA:
Prior to the extraction of DNA, frozen endodontic and periodontal specimens were suspended and allowed to settle at room temperature.The Qiamp DNA small kit (Qiagen, Hilden, Germany) was used for this experiment.The UV micro spectrophotometer 1240 was used to measure the concentration and purity the DNA.Through the use of an indirect evaluation method, the precise number of DNA copies within the bacterium was determined.The American Type Culture Collection (ATCC; Manassas, Virginia) provided Aggregatibacter actinomycetemcomitans ATCC 29523, Tannerella forsythia ATCC 43037, and Porphyromonas gingivalis ATCC 33277.Using the student's t test at a significance level of 0.05, the collected data was statistically assessed using the SPSS software programme, version 23.0.0 (SPSS, Chicago, Illinois).

Results:
Out of 50 patients in the current study, 22 were women and 28 were men.Table 1 lists the species found in the periodontium and endodontium.In 93% of the endodontium, 91% of the periodontium, 71%, and 48% of the P. gingivalis isolated from the endodontium and periodontium were found to harbour Tannerella forsythia.A. In 51% of the periodontium and 12% of the endodontium, Actinomycetemcomitans was identified.The total number of microorganisms found in the endodontium and periodontium for a particular tooth did not change significantly (Table 1).The presence of T. forsythia and P. gingivalis (r = 0.489 and P = 0.02), P. gingivalis and A. actinomycetemcomitans (r = 0.607 and P = 0.01), and T. forsythia and A. actinomycetemcomitans (r = 0.476 and P = 0.03) are all significantly correlated, according (

Conclusion:
Data shows that targeted bacterial species are associated with periodontal and endodontic disorders that occurred concurrently.Thus, dentinal tubules could serve as a conduit for bacterial dissemination.Hence, iatrogenic endodonticperiodontal lesions can be identified and treated more effectively with the use of a systematic diagnosis process.

[ 4 ]
. Permeability to the dentinal tubules can be influenced by various factors, including the size, adhesiveness, and motility of the bacteria [5].The dentinal tubule exposed to the periodontal ligament is covered by cement [6].Because the periodontium and endodontium are closely connected tissues, illnesses affecting one may affect the other.It can occasionally be challenging to differentiate between endodontic and periodontal illnesses, but getting the diagnosis right is essential to getting the right treatment [2, 7].The cornerstone of treatment for periodontal diseases is scaling and root planing, which involves removing contaminated cementum [4].The aetiology and accurate diagnosis of each unique illness determine the treatment and prognosis of endodontic-periodontal diseases [1].

Table 2 ).
[16]current investigation revealed that the number of targeted pathogens in the endodontium and periodontium of the same tooth did not differ statistically.The optimal natural conditions for bacterial development in periodontal pockets are provided by the significant quantifiable correlations of T. forsythia, F. nucleatum, and P. gingivalis quantification in these pockets, according to the results of the Pearson test.After comparing A. actinomycetemcomitans and T. forsythia, P. gingivalis and A. actinomycetemcomitans, and A. actinomycetemcomitans and T. forsythia, we found a fundamental connection between the bacteria in endodontic-peridontal disease.In patients having endodontic treatment with either mineral-trioxide or gutta-percha, the use of diode laser, the management of PRF and titanium-prepared PRF, and the deployment of bone grafts appear to be an adequate strategy[15].Iatrogenic endodontic-periodontal lesions can be identified and treated more effectively with the use of a systematic diagnosis process[16].The drawback of the present study is smaller sample size and use of only single rooted tooth.Further researches are needed to validate the findings.
[6]]odontal disease may be brought on by bacteria that enter the root canal through the apical foramen, according to Abbot and Salgado[10].By spreading through these channels or dentinal tubules, these bacteria may aid in the development of periodontitis[6].The frequency of Endo-Perio lesions